Reto Fiolka: "Improving the Spatio-temporal resolution in oblique plane microscopy"

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Speaker: Reto Fiolka (UT Southwestern Medical Center)

Abstract: Oblique plane microscopy combines the advantages of light-sheet illumination, such as low photo-toxicity, high sensitivity, and intrinsic optical sectioning, with the ease of use of a conventional epi-fluorescence microscope and the ability of high-speed volumetric imaging (Dunsby, Optics Express 2008, Bouchard, Nature Photonics 2015). With recent advances in sensitivity and resolution (Sapoznik, eLife 2020), OPM has become an emerging technology in the field of biological fluorescence imaging. Here I present recent developments in OPM to improve its spatial and temporal resolution. In oblique plane structured illumination microscopy (OPSIM), a structured light-sheet is launched out of a single, high numerical aperture objective. This light-sheet can be rotated along the optical axis, enabling multi-directional structured illumination in a light-sheet format. OPSIM achieves sub 150 nm lateral resolution and volume rates higher than 1 Hz. For high-speed volumetric interrogation, we have combined OPM with a multi-angle projection technique (Chang, Nature Methods 2021). Using iterative reconstruction algorithms, we can retrieve volumetric information from a small set of projections. Lastly, the projection technique can also be applied to OPSIM, yielding volumetric projection imaging at doubled resolution.

More information about Janelia’s Optical Interest Group can be found here: https://www.janelia.org/OIG
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